验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, human brain tissue, HeLa cells, Jurkat cells |
| Positive IHC detected in | human normal colon, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
产品信息
13508-1-AP targets MTPN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | MTPN fusion protein Ag4419 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myotrophin |
| 别名 | V 1, Protein V-1, Protein V 1, GCDP |
| 计算分子量 | 118 aa, 13 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC028093 |
| 基因名称 | MTPN |
| Gene ID (NCBI) | 136319 |
| RRID | AB_2148129 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P58546 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myotrophin (MTPN) is a ubiquitously expressed 12 kDa cytoplamic protein that was first isolated from the hearts of spontaneously hypertensive rats. Protein sequence analysis revealed that MTPN was composed of 118 amino acids and was occupied by two and a half internal 33 amino acid ankyrin repeats. MTPN stimulates protein synthesis and increases the expression of a number of cardiac marker genes (such as atrial natriuretic factor and b-myosin heavy chain) in cardiomyocytes leading to hypertrophy. In skeletal muscle, MTPN is also a positive growth factor in promoting skeletal muscle growth.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTPN antibody 13508-1-AP | Download protocol |
| IHC protocol for MTPN antibody 13508-1-AP | Download protocol |
| IF protocol for MTPN antibody 13508-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Obstet Gynecol Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression. | ||
J Clin Biochem Nutr Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction. |
