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验证数据展示

  • WB analysis of HeLa using 12299-1-AP

    WB analysis of HeLa using 12299-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 12299-1-AP

    IF Staining of A375 using 12299-1-AP

    Immunofluorescent analysis of A375 cells using 12299-1-AP (MTMR8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MTMR8 Polyclonal antibody

MTMR8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12299-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Phosphatidylinositol-3-phosphate phosphatase, Phosphatidylinositol-3,5-bisphosphate 3-phosphatase, Myotubularin-related protein 8, myotubularin related protein 8, EC:3.1.3.95

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A375 cells, human placenta tissue
Positive IF/ICC detected inA375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12299-1-AP targets MTMR8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2944

Product name: Recombinant human MTMR8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-307 aa of BC012399

Sequence: MDHITVPKVENVKLVDRYVSKKPANGILYLTATHLIYVEASGAARKETWIALHHIATVEKLPITSLGCPLTLRCKNFRVAHFVLDSDLVCHEVYISLLKLSQPALPEDLYAFSYNPKSSKEMRESGWKLIDPISDFGRMGIPNRNWTITDANRNYEICSTYPPEIVVPKSVTLGTVVGSSKFRSKERVPVLSYLYKENNAAICRCSQPLSGFYTRCVDDELLLEAISQTNPGSQFMYVVDTRPKLNAMANRAAGKGYENEDNYANIRFRFMGIENIHVMRSSLQKLLEVCELKTPTMSEFLSGLESS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myotubularin related protein 8
别名 Phosphatidylinositol-3-phosphate phosphatase, Phosphatidylinositol-3,5-bisphosphate 3-phosphatase, Myotubularin-related protein 8, myotubularin related protein 8, EC:3.1.3.95
计算分子量 704 aa, 79 kDa
观测分子量 55-58 kDa
GenBank蛋白编号BC012399
基因名称 MTMR8
Gene ID (NCBI) 55613
RRIDAB_2298075
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EF0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTMR8 antibody 12299-1-APDownload protocol
IF protocol for MTMR8 antibody 12299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols