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  • Featured Product
  • KD/KO Validated

MTMR14 Polyclonal antibody

MTMR14 Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 14973-1-AP

Print datasheet

Synonyms

C3orf29, hEDTP, HJUMPY, MTMR14, NS5ATP4ABP1

验证数据展示

  • WB analysis using 14973-1-AP

    WB analysis using 14973-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14973-1-AP (MTMR14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14973-1-AP

    IF Staining of HeLa using 14973-1-AP

    Immunofluorescent analysis of Hela cells, using MTMR14 antibody 14973-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HeLa using 14973-1-AP

    IP experiment of HeLa using 14973-1-AP

    IP result of anti-MTMR14 (IP:14973-1-AP, 4ug; Detection:14973-1-AP 1:500) with HeLa cells lysate 2000ug.

  • FC experiment of HeLa using 14973-1-AP

    FC experiment of HeLa using 14973-1-AP

    1X10^6 HeLa cells were stained with 0.2ug MTMR14 antibody (14973-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF detected inHela cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14973-1-AP targets MTMR14 in WB, IP, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen MTMR14 fusion protein Ag6883 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myotubularin related protein 14
Synonyms C3orf29, hEDTP, HJUMPY, MTMR14, NS5ATP4ABP1
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 110-120 kDa, 72 kDa
GenBank Accession NumberBC050626
Gene Symbol MTMR14
Gene ID (NCBI) 64419
RRIDAB_2147828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCE2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa, and also can be detected as a higher molecular weight due to the heterodimerization with other MTMR members.

实验方案

Product Specific Protocols
WB protocol for MTMR14 antibody 14973-1-APDownload protocol
IF protocol for MTMR14 antibody 14973-1-APDownload protocol
IP protocol for MTMR14 antibody 14973-1-APDownload protocol
FC protocol for MTMR14 antibody 14973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Gene

Knockdown MTMR14 promotes cell apoptosis and inhibits migration in liver cancer cells.

Authors - Zhaodong Li
  • KD Validated
View Article