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验证数据展示

  • WB analysis using 17552-1-AP

    WB analysis using 17552-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17552-1-AP

    WB analysis of HeLa using 17552-1-AP

    WB result of MTMR12 antibody (17552-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and MTMR12 knockout HeLa cells.

  • WB analysis of HEK-293 using 17552-1-AP

    WB analysis of HEK-293 using 17552-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 17552-1-AP

    IP experiment of K-562 using 17552-1-AP

    IP result of anti-MTMR12 (IP:17552-1-AP, 4ug; Detection:17552-1-AP 1:500) with K-562 cells lysate 2400 ug.

  • Featured Product
  • KD/KO验证

MTMR12 Polyclonal antibody

MTMR12 Polyclonal Antibody for WB, IP, ELISA
Cat No. 17552-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

KIAA1682, Inactive phosphatidylinositol 3-phosphatase 12, 3-phosphatase adapter protein, 3-PAP, 3 phosphatase adapter protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, mouse brain tissue, mouse lung tissue, HeLa cells
Positive IP detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17552-1-AP targets MTMR12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag11710

Product name: Recombinant human MTMR12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC057393

Sequence: MLGKGVVGGGGGTKAPKPSFVSYVRPEEIHTNEKEVTEKEVTLHLLPGEQLLCEASTVLKYVQEDSCQHGVYGRLVCTDFKIAFLGDDESALDNDETQFKNKVIGENDITLHCVDQIYGVFDEKKKTLFGQLKKYPEKLIIHCKDLRVFQFCLRYTKEEEVKRIVSGIIHHTQAPKLLKRLFLFSYATAAQNNTVTDPKNHTVMFDTLKDWCWELERTKGNMKYKAVSVNEGYKVCERLPAYFVVPTPLPEENVQRFQGHGIPIWCWSCHNGSALLKMSALPKEQDDGILQIQKSFLDGIYKTIHRPPYEIVKTEDLSSNFLSLQEIQTAYSKFKQLFLIDNSTEFWDTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myotubularin related protein 12
别名 KIAA1682, Inactive phosphatidylinositol 3-phosphatase 12, 3-phosphatase adapter protein, 3-PAP, 3 phosphatase adapter protein
计算分子量 86 kDa
观测分子量 73 kDa
GenBank蛋白编号BC057393
基因名称 MTMR12
Gene ID (NCBI) 54545
RRIDAB_2147824
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0I1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTMR12 antibody 17552-1-APDownload protocol
IP protocol for MTMR12 antibody 17552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

FASEB Bioadv

In search of alternatively spliced alpha-Klotho Kl1 protein in mouse brain

Authors - Liping Li
View Article
WB

BMC Urol

Effect of CTP-mediated PTEN on 5637 bladder cancer cells and the underlying molecular mechanism

Authors - Bei Yu
View Article