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验证数据展示

  • WB analysis using 32122-1-AP

    WB analysis using 32122-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32122-1-AP (MTHFSD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

MTHFSD Polyclonal antibody

MTHFSD Polyclonal Antibody for WB, ELISA
Cat No. 32122-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Methenyltetrahydrofolate Synthetase Domain-Containing Protein, Methenyltetrahydrofolate Synthetase Domain Containing, Methenyltetrahydrofolate synthase domain-containing protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HaCaT cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32122-1-AP targets MTHFSD in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MTHFSD fusion protein Ag37100 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methenyltetrahydrofolate synthetase domain containing
别名 Methenyltetrahydrofolate Synthetase Domain-Containing Protein, Methenyltetrahydrofolate Synthetase Domain Containing, Methenyltetrahydrofolate synthase domain-containing protein
观测分子量45 kDa
GenBank蛋白编号BC112059
基因名称 MTHFSD
Gene ID (NCBI) 64779
RRIDAB_3670199
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ2M296
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTHFSD is an RNA-binding protein that contains a methyltetrahydrofolate synthase structural domain and an RRM structural domain. In healthy motor neurons, MTHFSD is predominantly located in the nucleus, and studies have shown that MTHFSD, as a novel marker of stress granules, may be associated with aggregated proteins involved in amyotrophic lateral sclerosis (ALS).

实验方案

Product Specific Protocols
WB protocol for MTHFSD antibody 32122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols