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MTHFS Polyclonal antibody

MTHFS Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13114-1-AP

Print datasheet

Synonyms

HsT19268, Methenyl THF synthetase, MTHFS

验证数据展示

  • WB analysis using 13114-1-AP

    WB analysis using 13114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13114-1-AP

    IHC staining of human liver cancer using 13114-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver cancer using 13114-1-AP

    IHC staining of human liver cancer using 13114-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, human liver tissue, Jurkat cells, human stomach tissue, U-937 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13114-1-AP targets MTHFS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen MTHFS fusion protein Ag3781 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)
Synonyms HsT19268, Methenyl THF synthetase, MTHFS
Calculated Molecular Weight 203 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC019921
Gene Symbol MTHFS
Gene ID (NCBI) 10588
RRIDAB_2250977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTHFS antibody 13114-1-APDownload protocol
IHC protocol for MTHFS antibody 13114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols