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验证数据展示

  • WB analysis using 13114-1-AP

    WB analysis using 13114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 13114-1-AP

    IP experiment of HeLa using 13114-1-AP

    IP result of anti-MTHFS (IP:13114-1-AP, 4ug; Detection:13114-1-AP 1:2000) with HeLa cells lysate 1200 ug.

  • IHC staining of human liver cancer using 13114-1-AP

    IHC staining of human liver cancer using 13114-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver cancer using 13114-1-AP

    IHC staining of human liver cancer using 13114-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 40x lens).

MTHFS Polyclonal antibody

MTHFS Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13114-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

Methenyl-THF synthetase, Methenyl THF synthetase, HsT19268, EC:6.3.3.2, 5-formyltetrahydrofolate cyclo-ligase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, human liver tissue, Jurkat cells, human stomach tissue, U-937 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13114-1-AP targets MTHFS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3781

Product name: Recombinant human MTHFS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-203 aa of BC019921

Sequence: MAAAAVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQDEIETEEIIKDIFQRGKICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQPGEGDVREEALSTGGLDLIFMPGLGFDKHGNRLGRGKGYYDAYLKRCLQHQEVKPYTLALAFKEQICLQVPVNENDMKVDEVLYEDSSTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)
别名 Methenyl-THF synthetase, Methenyl THF synthetase, HsT19268, EC:6.3.3.2, 5-formyltetrahydrofolate cyclo-ligase
计算分子量 203 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC019921
基因名称 MTHFS
Gene ID (NCBI) 10588
RRIDAB_2250977
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49914
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTHFS antibody 13114-1-APDownload protocol
IHC protocol for MTHFS antibody 13114-1-APDownload protocol
IP protocol for MTHFS antibody 13114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols