• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84884-5-RR

    WB analysis using 84884-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84884-5-RR (MTHFD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 84884-5-RR

    IHC staining of human liver cancer using 84884-5-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84884-5-RR (MTHFD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTHFD1 antibody included
  • IHC staining of human liver cancer using 84884-5-RR

    IHC staining of human liver cancer using 84884-5-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84884-5-RR (MTHFD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTHFD1 antibody included
  • IHC staining of human colon cancer using 84884-5-RR

    IHC staining of human colon cancer using 84884-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84884-5-RR (MTHFD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTHFD1 antibody included
  • IHC staining of human colon cancer using 84884-5-RR

    IHC staining of human colon cancer using 84884-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84884-5-RR (MTHFD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTHFD1 antibody included
  • IF Staining of HEK-293 using 84884-5-RR

    IF Staining of HEK-293 using 84884-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MTHFD1 antibody (84884-5-RR, Clone: 242388F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84884-5-RR

    Affinity and Kinetic Characterization of 84884-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84884-5-RR against Human MTHFD1 were performed. The affinity constant is below 1 pM.

MTHFD1 Recombinant monoclonal antibody

MTHFD1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84884-5-RR

产品说明书

CloneNo. 242388F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

242388F1, C-1-tetrahydrofolate synthase, cytoplasmic, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C1-THF synthase, Epididymis secretory sperm binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inK-562 cells, HuH-7 cells, HEK-293 cells, A549 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MTHFD1 Ready-To-Use IHC Kit. MTHFD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84884-5-RR targets MTHFD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1225

Product name: Recombinant human MTHFD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 536-935 aa of BC009806

Sequence: TNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADQIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
别名 242388F1, C-1-tetrahydrofolate synthase, cytoplasmic, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C1-THF synthase, Epididymis secretory sperm binding protein
计算分子量 101 kDa
观测分子量 101-110 kDa
GenBank蛋白编号BC009806
基因名称 MTHFD1
Gene ID (NCBI) 4522
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP11586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

实验方案

Product Specific Protocols
IF protocol for MTHFD1 antibody 84884-5-RRDownload protocol
IHC protocol for MTHFD1 antibody 84884-5-RRDownload protocol
WB protocol for MTHFD1 antibody 84884-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...