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验证数据展示

  • WB analysis using 17778-1-AP

    WB analysis using 17778-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17778-1-AP (MTFR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 17778-1-AP

    WB analysis of A549 using 17778-1-AP

    WB result of MTFR1 antibody (17778-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTFR1 transfected A549 cells.

  • IP experiment of mouse testis using 17778-1-AP

    IP experiment of mouse testis using 17778-1-AP

    IP result of anti-MTFR1 (IP:17778-1-AP, 4ug; Detection:17778-1-AP 1:800) with mouse testis tissue lysate 4800ug.

  • Featured Product
  • KD/KO验证

MTFR1 Polyclonal antibody

MTFR1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 17778-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IP, ELISA

别名

CHPPR, FAM54A2, KIAA0009, MTFR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HCT 116 cells, HepG2 cells, SW480 cells
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17778-1-AP targets MTFR1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性monkey
免疫原

CatNo: Ag10857

Product name: Recombinant human MTFR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-333 aa of BC036116

Sequence: MLGWIKRLIRMVFQQVGVSMQSVLWSRKPYGSSRSIVRKIGTNLSLIQCPRVQFQINSHATEWSPSHPGEDAVASFADVGWVAKEEGECSARLRTEVRSRPPLQDDLLFFEKAPSRQISLPDLSQEEPQLKTPARANEEALQKICALENELAALRAQIAKIVTQQEQQNLTAGDLDSTTFGTIPPHPPPPPPPLPPPALGLHQSTSAVDLIKERREKRANAGKTLVKNNPKKPEMPNMLEILKEMNSVKLRSVKRSEQDVKPKPVDATDPAALIAEALKKKFAYRYRSDSQDEVEKGIPKSESEATSERVLFGPHMLKPTGKMKALIENVSDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial fission regulator 1
别名 CHPPR, FAM54A2, KIAA0009, MTFR1
计算分子量 333 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC036116
基因名称 MTFR1
Gene ID (NCBI) 9650
RRIDAB_2282301
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15390
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTFR1, also known as CHPPR, is a mitochondria protein. It may play a role in mitochondrial aerobic respiration. It may also regulate mitochondrial organization and fission.

实验方案

Product Specific Protocols
WB protocol for MTFR1 antibody 17778-1-APDownload protocol
IP protocol for MTFR1 antibody 17778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
monkeyWB

Sci Adv

ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution

Authors - Michael J McKenna
View Article