验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, mouse ES cells and 293 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | mouse ES cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16208-1-AP targets MTF2 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MTF2 fusion protein Ag9182 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | metal response element binding transcription factor 2 |
| 别名 | PCL2, MTF 2, Metal-response element-binding transcription factor 2, Metal-response element DNA-binding protein M96, M96 |
| 计算分子量 | 536 aa, 61 kDa |
| 观测分子量 | 67 kDa, 60 kDa, 60-61 kDa |
| GenBank蛋白编号 | BC010013 |
| 基因名称 | MTF2 |
| Gene ID (NCBI) | 22823 |
| RRID | AB_2147370 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y483 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTF2 antibody 16208-1-AP | Download protocol |
| IHC protocol for MTF2 antibody 16208-1-AP | Download protocol |
| IP protocol for MTF2 antibody 16208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding. | ||
Nat Genet RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells. | ||
Nat Cell Biol Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes | ||
Mol Cell Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells. |
