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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 31892-1-AP

    WB analysis using 31892-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31892-1-AP (MTERFD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MTERF4 Polyclonal antibody

MTERF4 Polyclonal Antibody for WB, ELISA
Cat No. 31892-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

MTERFD2, Transcription termination factor 4, mitochondrial, mTERF domain-containing protein 2, MTERF Domain Containing 2 , Mitochondrial transcription termination factor 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, LNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31892-1-AP targets MTERF4 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MTERF4 fusion protein Ag32334 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MTERF domain containing 2
别名 MTERFD2, Transcription termination factor 4, mitochondrial, mTERF domain-containing protein 2, MTERF Domain Containing 2 , Mitochondrial transcription termination factor 4
计算分子量44 kDa
观测分子量37 kDa
GenBank蛋白编号NM_182501
基因名称 MTERFD2
Gene ID (NCBI) 130916
RRIDAB_3670135
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ7Z6M4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MTERF protein family plays a key role in controlling mitochondrial DNA gene expression in mammals. Mitochondrial transcription termination factor 4 (MTERF4, also known as MTERFD2) regulates mitochondrial ribosome biogenesis and translation (PMID: 21531335). It can form a stoichiometric complex with the ribosomal RNA methyl-transferase NSUN4 and is necessary for the recruitment of this factor to the large ribosomal subunit. Loss of MTERF4 leads to defective ribosomal assembly and a drastic reduction in translation (PMID: 21531335).

实验方案

Product Specific Protocols
WB protocol for MTERF4 antibody 31892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols