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验证数据展示

  • IF Staining of U2OS using 10235-1-AP

    IF Staining of U2OS using 10235-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MTCP1NB antibody (10235-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IP experiment of HEK-293T using 10235-1-AP

    IP experiment of HEK-293T using 10235-1-AP

    IP result of anti-MTCP1NB (IP:10235-1-AP, 4ug; Detection:10235-1-AP 1:300) with HEK-293T cells lysate 1320 ug.

MTCP1NB Polyclonal antibody

MTCP1NB Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 10235-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, IP, ELISA

别名

MTCP 1 type A, Mature T-cell proliferation-1 type A, Mature T-cell proliferation 1 neighbor protein, Cx9C motif-containing protein 4, CMC4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHEK-293T cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10235-1-AP targets MTCP1NB in IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 MTCP1NB fusion protein Ag0338 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mature T-cell proliferation 1 neighbor
别名 MTCP 1 type A, Mature T-cell proliferation-1 type A, Mature T-cell proliferation 1 neighbor protein, Cx9C motif-containing protein 4, CMC4
计算分子量 13 kDa, 8 kDa
观测分子量 8 kDa
GenBank蛋白编号BC002600
基因名称 MTCP1NB
Gene ID (NCBI) 100272147
RRIDAB_2189123
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56277
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTCP1NB, also named as C6.1B, MTCP1, MTCP1B, is a member of the CMC4 protein family. MTCP1NB is 8 kDa protein that localizes to mitochondria. MTCP1NB was identified by involvement in some t(X;14) translocations associated with mature T-cell proliferations(NCBI).

实验方案

Product Specific Protocols
IF protocol for MTCP1NB antibody 10235-1-APDownload protocol
IP protocol for MTCP1NB antibody 10235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols