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验证数据展示

  • WB analysis of HEK-293 using 83005-1-RR (same clone as 83005-1-PBS)

    WB analysis of HEK-293 using 83005-1-RR (same clone as 83005-1-PBS)

    WB result of MTAP antibody (83005-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTAP transfected HEK-293 cells. This data was developed using the same antibody clone with 83005-1-PBS in a different storage buffer formulation.

  • WB analysis using 83005-1-RR (same clone as 83005-1-PBS)

    WB analysis using 83005-1-RR (same clone as 83005-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83005-1-RR (MTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83005-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83005-1-RR

    Affinity and Kinetic Characterization of 83005-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83005-1-RR against Human MTAP were performed. The affinity constant is 0.782 nM.

  • Featured Product
  • KD/KO验证
    • {{ptg:ProductFeaturesInfo}}

MTAP Recombinant monoclonal antibody, PBS Only

MTAP Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 83005-1-PBS

产品说明书

CloneNo. 240030A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

240030A3, 5'-methylthioadenosine phosphorylase, c86fus, EC:2.4.2.28, MSAP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83005-1-PBS targets MTAP in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2051

Product name: Recombinant human MTAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-154 aa of BC018625

Sequence: MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCILLARHGRQHTIMPSKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDSHVRRACFPFTFHHDCFQRPPQKPSRSHCVSCATCRTMS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methylthioadenosine phosphorylase
别名 240030A3, 5'-methylthioadenosine phosphorylase, c86fus, EC:2.4.2.28, MSAP
计算分子量 283 aa, 31 kDa
观测分子量31 kDa
GenBank蛋白编号BC018625
基因名称 MTAP
Gene ID (NCBI) 4507
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13126
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.