MTAP Monoclonal antibody

MTAP Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66706-1-Ig

产品说明书

CloneNo. 2B1G6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inNIH/3T3 cells, HCT 116 cells, HSC-T6 cells, Raji cells, HT-29 cells
Positive IHC detected inmouse liver tissue, human kidney tissue, rat liver tissue, mouse kidney tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66706-1-Ig targets MTAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 MTAP fusion protein Ag17954 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 methylthioadenosine phosphorylase
别名 c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase
计算分子量 283 aa, 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC018625
基因名称 MTAP
Gene ID (NCBI) 4507
RRIDAB_2882058
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13126
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

实验方案

Product Specific Protocols
WB protocol for MTAP antibody 66706-1-IgDownload protocol
IHC protocol for MTAP antibody 66706-1-IgDownload protocol
IF protocol for MTAP antibody 66706-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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