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- KD/KO Validated
MTAP Polyclonal antibody
MTAP Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Raji cells, mouse heart tissue, human heart tissue, NIH/3T3 cells, HeLa cells, HT-29 cells, C6 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11475-1-AP targets MTAP in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, rat |
| Immunogen | MTAP fusion protein Ag2051 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | methylthioadenosine phosphorylase |
| Synonyms | c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase |
| Calculated Molecular Weight | 283 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC018625 |
| Gene Symbol | MTAP |
| Gene ID (NCBI) | 4507 |
| RRID | AB_2147094 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13126 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTAP antibody 11475-1-AP | Download protocol |
| IHC protocol for MTAP antibody 11475-1-AP | Download protocol |
| IF protocol for MTAP antibody 11475-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest SHARPIN-mediated regulation of protein arginine methyltransferase 5 controls melanoma growth. | ||
Nat Commun MTAP deficiency creates an exploitable target for antifolate therapy in 9p21-loss cancers. | ||
Nat Commun Pharmacological polyamine catabolism upregulation with methionine salvage pathway inhibition as an effective prostate cancer therapy. | ||
Clin Cancer Res Homozygous deletion of MTAP gene as a poor prognosticator in gastrointestinal stromal tumors. | ||
Oncoimmunology Suppressive effects of tumor cell-derived 5'-deoxy-5'-methylthioadenosine on human T cells. | ||
J Pathol Methylthioadenosine phosphorylase and activated insulin-like growth factor-1 receptor/insulin receptor: potential therapeutic targets in chordoma. |
