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验证数据展示

  • WB analysis using 87979-1-RR

    WB analysis using 87979-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87979-1-RR (MTA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 87979-1-RR

    IF Staining of HeLa using 87979-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTA1 antibody (87979-1-RR, Clone: 253519C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87979-1-RR

    Affinity of 87979-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87979-1-RR against Human MTA1 were performed. The affinity constant is 7.08 nM.

MTA1 Recombinant monoclonal antibody

MTA1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87979-1-RR

产品说明书

CloneNo. 253519C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

metastasis associated 1, MTA 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, MCF-7 cells, Jurkat cells, A549 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy MTA1 Ready-To-Use IHC Kit. MTA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87979-1-RR targets MTA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30994

Product name: Recombinant human MTA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 513-678 aa of NM_004689

Sequence: TARLPEASQSPLVLKQAVRKPLEAVLRYLETHPRPPKPDPVKSVSSVLSSLTPAKVAPVINNGSPTILGKRSYEQHNGVDGNMKKRLLMPSRGLANHGQARHMGPSRNLLLNGKSYPTKVRLIRGGSLPPVKRRRMNWIDAPDDVFYMATEETRKIRKLLSSSETK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 metastasis associated 1
别名 metastasis associated 1, MTA 1
计算分子量 81 kDa
观测分子量70-80 kDa
GenBank蛋白编号NM_004689
基因名称 MTA1
Gene ID (NCBI) 9112
RRIDAB_3745844
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13330
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Metastasis-associated protein 1 (MTA1) is a member of the MTA family, which is involved in regulating DNA-based biological processes (PMID: 25332144). MTA1 is an integral part of the nucleosome remodeling histone deacetylase complex (NuRD complex) and regulates nucleosome remodeling and deacetylation by stabilizing the NuRD complex and related proteins (PMID: 12419236). MTA1 expression is associated with tumor malignancy in several cancers.

实验方案

Product Specific Protocols
IF protocol for MTA1 antibody 87979-1-RRDownload protocol
WB protocol for MTA1 antibody 87979-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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