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验证数据展示

  • WB analysis using 28400-1-AP

    WB analysis using 28400-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28400-1-AP (MSTO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 28400-1-AP

    WB analysis of HepG2 using 28400-1-AP

    WB result of MSTO1 antibody (28400-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSTO1 transfected HepG2 cells.

  • IHC staining of human ovary cancer using 28400-1-AP

    IHC staining of human ovary cancer using 28400-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28400-1-AP (MSTO1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO验证

MSTO1 Polyclonal antibody

MSTO1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28400-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

SLTP005, MST, MMYAT, LST005

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28400-1-AP targets MSTO1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29067

Product name: Recombinant human MSTO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 28-256 aa of BC002535

Sequence: QDAALGRATDSKEPPGELCPDVLYRTGRTLHGQETYTPRLILMDLKGSLSSLKEEGGLYRDKQLDAAIAWQGKLTTHKEELYPKNPYLQDFLSAEGVLSSDGVWRVKSIPNGKGSSPLPTATTPKPLIPTEASIRVWSDFLRVHLHPRSICMIQKYNHDGEAGRLEAFGQGESVLKEPKYQEELEDRLHFYVEECDYLQGFQILCDLHDGFSGVGAKAAELLQDEYSGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 misato homolog 1 (Drosophila)
别名 SLTP005, MST, MMYAT, LST005
观测分子量 57-62 kDa
GenBank蛋白编号BC002535
基因名称 MSTO1
Gene ID (NCBI) 55154
RRIDAB_2881133
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUK6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSTO1, also named as LST005 and SLTP005, belongs to the misato family. Human MSTO1 is a ubiquitously expressed, evolutionary conserved protein with homologous regions to GTPases. MSTO1 is a cytoplasmic protein that modulates mitochondrial dynamics by promoting mitochondrial fusion. MSTO1 has 7 isoforms produced by alternative splicing. (PMID: 33612823)

实验方案

Product Specific Protocols
IHC protocol for MSTO1 antibody 28400-1-APDownload protocol
WB protocol for MSTO1 antibody 28400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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