验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse liver tissue, PC-3 cells, mouse heart tissue |
| Positive IHC detected in | human heart tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19142-1-AP targets GDF8/Myostatin in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, zebrafish, bovine, sheep, goat |
| 免疫原 |
CatNo: Ag13605 Product name: Recombinant human MSTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-375 aa of BC074757 Sequence: LNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myostatin |
| 别名 | GDF8, MSTN, myostatin, GDF 8, GDF-8 |
| 计算分子量 | 375 aa, 43 kDa |
| 观测分子量 | 50 and 35 kDa |
| GenBank蛋白编号 | BC074757 |
| 基因名称 | MSTN |
| Gene ID (NCBI) | 2660 |
| RRID | AB_10638615 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14793 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myostatin, also known as GDF-8, is a member of the TGF-beta superfamily. Myostatin is expressed in the myotome and developing skeletal muscles. Myostatin negatively regulates skeletal muscle growth and development through the regulation of anabolic and catabolic pathways in skeletal muscles. Myostatin immunoreactive bands could be identified at ∼50, 32-35, and 16 kDa, which correspond to the precursor, glycosylated dimmer, and monomer, respectively (PMID: 17711997). GDF8 may have cross-reactions with GDF11.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GDF8/Myostatin antibody 19142-1-AP | Download protocol |
| IHC protocol for GDF8/Myostatin antibody 19142-1-AP | Download protocol |
| WB protocol for GDF8/Myostatin antibody 19142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol Muscle-homing peptides modified biomimetic curcumin nanoparticles ameliorate skeletal muscle dysfunction in aging mice | ||
Adv Sci (Weinh) Blockade of Activin Receptor IIB Protects Arthritis Pathogenesis by Non-Amplification of Activin A-ACVR2B-NOX4 Axis Pathway | ||
Cell Death Dis Endurance exercise remodels skeletal muscle by suppressing Ythdf1-mediated myostatin expression | ||
Mater Today Bio Muscle homing peptide modified liposomes loaded with EGCG improved skeletal muscle dysfunction by inhibiting inflammation in aging mice | ||
J Cachexia Sarcopenia Muscle MyoMed205 Counteracts Titin Hyperphosphorylation and the Expression of Contraction-Regulating Proteins in a Rat Model of HFpEF | ||
J Cachexia Sarcopenia Muscle Myostatin Exhibits an Evolutionarily Conserved Circadian Pattern in Skeletal Muscles. |
