验证数据展示
经过测试的应用
Positive WB detected in | mouse lung tissue |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
11053-1-AP targets RON, MST1R in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | RON, MST1R fusion protein Ag1537 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | macrophage stimulating 1 receptor (c-met-related tyrosine kinase) |
别名 | CD136, CDw136, MSP receptor, MST1R, p185 Ron, Protein tyrosine kinase 8, PTK8, RON, RON, MST1R |
计算分子量 | 152 kDa |
观测分子量 | 140 kDa |
GenBank蛋白编号 | BQ936677 NM_002447 |
基因名称 | RON |
Gene ID (NCBI) | 4486 |
RRID | AB_874180 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q04912 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RON (also referred to as MST1R, or CD136) is a receptor tyrosine kinase that belongs to the MET proto-oncogene family. The precursor RON is produced as a glycosylated, single chain that is cleaved into a 185-kDa heterodimer of α (35 kDa) and β (150 kDa) subunits connected by a disulfide bond (PMID: 8062829). It is activated upon binding to its ligand, macrophage-stimulating protein (MSP, also known as MST1 and HGFL). RON signalling is essential for embryonic development and regulates many physiological processes including cell survival, migration and differentiation.
实验方案
Product Specific Protocols | |
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WB protocol for RON, MST1R antibody 11053-1-AP | Download protocol |
IHC protocol for RON, MST1R antibody 11053-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Ther Oncolytics RUNX1/EGFR pathway contributes to STAT3 activation and tumor growth caused by hyperactivated mTORC1. | ||
Theranostics Development of dual aptamers-functionalized c-MET PROTAC degraders for targeted therapy of osteosarcoma |