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RON, MST1R Polyclonal antibody

RON, MST1R Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11053-1-AP

Print datasheet

Synonyms

CD136, CDw136, MSP receptor, MST1R, p185 Ron, Protein tyrosine kinase 8, PTK8, RON, RON, MST1R

验证数据展示

  • WB analysis of mouse lung using 11053-1-AP

    WB analysis of mouse lung using 11053-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11053-1-AP (RON, MST1R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11053-1-AP

    IHC staining of human lung cancer using 11053-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11053-1-AP

    IHC staining of human lung cancer using 11053-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11053-1-AP targets RON, MST1R in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RON, MST1R fusion protein Ag1537 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Synonyms CD136, CDw136, MSP receptor, MST1R, p185 Ron, Protein tyrosine kinase 8, PTK8, RON, RON, MST1R
Calculated Molecular Weight 152 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBQ936677 NM_002447
Gene Symbol MST1R
Gene ID (NCBI) 4486
RRIDAB_874180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04912
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RON (also referred to as MST1R, or CD136) is a receptor tyrosine kinase that belongs to the MET proto-oncogene family. The precursor RON is produced as a glycosylated, single chain that is cleaved into a 185-kDa heterodimer of α (35 kDa) and β (150 kDa) subunits connected by a disulfide bond (PMID: 8062829). It is activated upon binding to its ligand, macrophage-stimulating protein (MSP, also known as MST1 and HGFL). RON signalling is essential for embryonic development and regulates many physiological processes including cell survival, migration and differentiation.

实验方案

Product Specific Protocols
WB protocol for RON, MST1R antibody 11053-1-APDownload protocol
IHC protocol for RON, MST1R antibody 11053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Ther Oncolytics

RUNX1/EGFR pathway contributes to STAT3 activation and tumor growth caused by hyperactivated mTORC1.

Authors - Wei Lin
View Article