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Moesin Polyclonal antibody

Moesin Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26053-1-AP

Print datasheet

Synonyms

moesin, MSN

验证数据展示

  • WB analysis of Jurkat using 26053-1-AP

    WB analysis of Jurkat using 26053-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26053-1-AP

    IF Staining of HeLa using 26053-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26053-1-AP (MSN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse kidney using 26053-1-AP

    IHC staining of mouse kidney using 26053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26053-1-AP (MSN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 26053-1-AP

    IHC staining of mouse kidney using 26053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26053-1-AP (MSN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 26053-1-AP

    FC experiment of HepG2 using 26053-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.20ug Anti-Human MSN (26053-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

  • WB analysis using 26053-1-AP

    WB analysis using 26053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, Neuro-2a cells, C6 cells, mouse heart tissue, mouse lung tissue, rat heart tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26053-1-AP targets Moesin in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Moesin fusion protein Ag23531 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name moesin
Synonyms moesin, MSN
Calculated Molecular Weight 577 aa, 68 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC017293
Gene Symbol MSN
Gene ID (NCBI) 4478
RRIDAB_2880353
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26038
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

实验方案

Product Specific Protocols
WB protocol for Moesin antibody 26053-1-APDownload protocol
IHC protocol for Moesin antibody 26053-1-APDownload protocol
IF protocol for Moesin antibody 26053-1-APDownload protocol
FC protocol for Moesin antibody 26053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols