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验证数据展示

  • IHC staining of human ovary tumor using 15600-1-AP

    IHC staining of human ovary tumor using 15600-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 15600-1-AP

    IHC staining of human ovary tumor using 15600-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Mesothelin Polyclonal antibody

Mesothelin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15600-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CAK1, CAK1 antigen, mesothelin, MPF, MPF Mesothelin, cleaved form, MSLN, SMR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman ovary tumor tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15600-1-AP targets Mesothelin in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Mesothelin fusion protein Ag7981 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mesothelin
别名 CAK1, CAK1 antigen, mesothelin, MPF, MPF Mesothelin, cleaved form, MSLN, SMR
计算分子量 69 kDa
GenBank蛋白编号BC003512
基因名称 MSLN
Gene ID (NCBI) 10232
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13421
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).

实验方案

Product Specific Protocols
IHC protocol for Mesothelin antibody 15600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols