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验证数据展示

  • WB analysis using 23601-1-AP

    WB analysis using 23601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23601-1-AP (MSL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 23601-1-AP

    IF Staining of A431 using 23601-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSL2 antibody (23601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

MSL2 Polyclonal antibody

MSL2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23601-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

MS L2, MSL 2, MSL2 like 1, MSL2L1, RNF184

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23601-1-AP targets MSL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18727

Product name: Recombinant human MSL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 228-577 aa of BC093790

Sequence: TEDLSDSLPPVCDTVATDLCSTGIDICSFSEDIKPGDSLLLSVEEVLRSLETVSNTEVCCPNLQPNLEATVSNGPFLQLSSQSLSHNVFMSTSPALHGLSCTAATPKIAKLNRKRSRSESDSEKVQPLPISTIIRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSHSKTKPGILKKDKAVKEKIPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQRCPCYSNRKACLDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNASTSTSVINVTGSPVTTFLAASTHDDKSLDEAIDMRFDC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 male-specific lethal 2 homolog (Drosophila)
别名 MS L2, MSL 2, MSL2 like 1, MSL2L1, RNF184
计算分子量 577 aa, 63 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC093790
基因名称 MSL2
Gene ID (NCBI) 55167
RRIDAB_3742087
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9HCI7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In mammals, MSL2 participates in histone H2B ubiquitination (H2Bub1) via the MSL complex, regulating transcription and chromatin remodeling. It is essential for embryonic development and has been implicated in cancer progression. MSL2's dysfunction is linked to developmental defects and oncogenesis, highlighting its critical role in gene regulation. (PMID: 30669413; PMID: 34899909)

实验方案

Product Specific Protocols
IF protocol for MSL2 antibody 23601-1-APDownload protocol
WB protocol for MSL2 antibody 23601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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