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验证数据展示

  • WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

    WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86723-1-RR (MSI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

  • WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

    WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86723-1-RR (MSI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse embryo using 86723-1-RR (same clone as 86723-1-PBS)

    IHC staining of mouse embryo using 86723-1-RR (same clone as 86723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 86723-1-RR (MSI1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSI1 antibody included
  • Cytometric bead array sample test of MP02619-1

    Cytometric bead array sample test of MP02619-1

    The mean MSI1 concentration was determined to be 12.9 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 9.0 ng/mL in Caco-2 cell extract based on a 1.3 mg/mL extract load, 20.7 ng/mL in HEK-293T cell extract based on a 2.6 mg/mL extract load.

  • Cytometric bead array standard curve of MP02619-1

    Cytometric bead array standard curve of MP02619-1

    Cytometric bead array standard curve of MP02619-1, MSI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86723-1-PBS. Detection antibody: 86723-2-PBS. Standard: Ag26042. Range: 0.312-40 ng/mL.

  • Affinity and Kinetic Characterization of 86723-1-RR

    Affinity and Kinetic Characterization of 86723-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86723-1-RR against Human MSI1 were performed. The affinity constant is 93.8 pM.

MSI1 Recombinant monoclonal antibody, PBS Only (Capture)

MSI1 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 86723-1-PBS

产品说明书

CloneNo. 251695G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Musashi 1, musashi homolog 1 (Drosophila), Musashi-1, RNA-binding protein Musashi homolog 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MSI1 Ready-To-Use IHC Kit. MSI1 primary antibody included.

产品信息

86723-1-PBS targets MSI1 as part of a matched antibody pair:

MP02619-1: 86723-1-PBS capture and 86723-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26042

Product name: Recombinant human MSI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-83 aa of BC146463

Sequence: METDAPQPGLASPDSPHDPCKMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 musashi homolog 1 (Drosophila)
别名 Musashi 1, musashi homolog 1 (Drosophila), Musashi-1, RNA-binding protein Musashi homolog 1
计算分子量 362 aa, 39 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC146463
基因名称 MSI1
Gene ID (NCBI) 4440
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43347
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.

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