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验证数据展示

  • WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

    WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86723-1-RR (MSI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

  • WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

    WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86723-1-RR (MSI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse embryo using 86723-1-RR (same clone as 86723-1-PBS)

    IHC staining of mouse embryo using 86723-1-RR (same clone as 86723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 86723-1-RR (MSI1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSI1 antibody included
  • IF Staining of SH-SY5Y using 86723-1-RR (same clone as 86723-1-PBS)

    IF Staining of SH-SY5Y using 86723-1-RR (same clone as 86723-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MSI1 antibody (86723-1-RR, Clone: 251695G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02619-1

    Cytometric bead array sample test of MP02619-1

    The mean MSI1 concentration was determined to be 12.9 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 9.0 ng/mL in Caco-2 cell extract based on a 1.3 mg/mL extract load, 20.7 ng/mL in HEK-293T cell extract based on a 2.6 mg/mL extract load.

  • Cytometric bead array standard curve of MP02619-1

    Cytometric bead array standard curve of MP02619-1

    Cytometric bead array standard curve of MP02619-1, MSI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86723-1-PBS. Detection antibody: 86723-2-PBS. Standard: Ag26042. Range: 0.312-40 ng/mL.

  • Affinity and Kinetic Characterization of 86723-1-RR

    Affinity and Kinetic Characterization of 86723-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86723-1-RR against Human MSI1 were performed. The affinity constant is 93.8 pM.

MSI1 Recombinant monoclonal antibody, PBS Only (Capture)

MSI1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86723-1-PBS

产品说明书

CloneNo. 251695G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Musashi 1, musashi homolog 1 (Drosophila), Musashi-1, RNA-binding protein Musashi homolog 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MSI1 Ready-To-Use IHC Kit. MSI1 primary antibody included.

产品信息

86723-1-PBS targets MSI1 as part of a matched antibody pair:

MP02619-1: 86723-1-PBS capture and 86723-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26042

Product name: Recombinant human MSI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-83 aa of BC146463

Sequence: METDAPQPGLASPDSPHDPCKMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 musashi homolog 1 (Drosophila)
别名 Musashi 1, musashi homolog 1 (Drosophila), Musashi-1, RNA-binding protein Musashi homolog 1
计算分子量 362 aa, 39 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC146463
基因名称 MSI1
Gene ID (NCBI) 4440
RRIDAB_3745079
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43347
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.

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