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验证数据展示

  • WB analysis using 82775-1-RR (same clone as 82775-1-PBS)

    WB analysis using 82775-1-RR (same clone as 82775-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82775-1-RR (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82775-1-RR (same clone as 82775-1-PBS)

    WB analysis of HeLa using 82775-1-RR (same clone as 82775-1-PBS)

    WB result of MSH2 antibody (82775-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH2 transfected HeLa cells. This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82775-1-RR (same clone as 82775-1-PBS)

    IF Staining of HeLa using 82775-1-RR (same clone as 82775-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH2 antibody (82775-1-RR, Clone: 6O4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82775-1-RR (same clone as 82775-1-PBS)

    IHC staining of human colon cancer using 82775-1-RR (same clone as 82775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82775-1-RR (MSH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human colon using 82775-1-RR (same clone as 82775-1-PBS)

    IHC staining of human colon using 82775-1-RR (same clone as 82775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82775-1-RR (MSH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human colon using 82775-1-RR (same clone as 82775-1-PBS)

    IHC staining of human colon using 82775-1-RR (same clone as 82775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82775-1-RR (MSH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human tonsillitis using 82775-1-RR (same clone as 82775-1-PBS)

    IHC staining of human tonsillitis using 82775-1-RR (same clone as 82775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82775-1-RR (MSH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • Affinity and Kinetic Characterization of 82775-1-RR

    Affinity and Kinetic Characterization of 82775-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82775-1-RR against Human MSH2 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

MSH2 Recombinant monoclonal antibody, PBS Only

MSH2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82775-1-PBS

产品说明书

CloneNo. 6O4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

6O4, MSH 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.

产品信息

82775-1-PBS targets MSH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7835

Product name: Recombinant human MSH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 585-934 aa of BC021566

Sequence: SSGYVEPMQTLNDVLAQLDAVVSFAHVSNGAPVPYVRPAILEKGQGRIILKASRHACVEVQDEIAFIPNDVYFEKDKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGCFVPCESAEVSIVDCILARVGAGDSQLKGVSTFMAEMLETASILRSATKDSLIIIDELGRGTSTYDGFGLAWAISEYIATKIGAFCMFATHFHELTALANQIPTVNNLHVTALTTEETLTMLYQVKKGVCDQSFGIHVAELANFPKHVIECAKQKALELEEFQYIGESQGYDIMEPAAKKCYLEREQGEKIIQEFLSKVKQMPFTEMSEENITIKLKQLKAEVIAKNNSFVNEIISRIKVTT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
别名 6O4, MSH 2
计算分子量 105 kDa
观测分子量 100 kDa-105 kDa
GenBank蛋白编号BC021566
基因名称 MSH2
Gene ID (NCBI) 4436
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP43246
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

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