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验证数据展示

  • WB analysis using 60161-2-Ig

    WB analysis using 60161-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60161-2-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of Ramos using 60161-2-Ig

    IHC staining of Ramos using 60161-2-Ig

    Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human tonsillitis using 60161-2-Ig

    IHC staining of human tonsillitis using 60161-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH2 antibody included

MSH2 Monoclonal antibody

MSH2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60161-2-Ig

产品说明书

CloneNo. 2E4A7

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, ELISA

别名

2E4A7, MSH 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, A431 cells, HuT 78 cells, MOLT-4 cells, K-562 cells
Positive IHC detected inRamos cells, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60161-2-Ig targets MSH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 MSH2 fusion protein Ag7835 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
别名 2E4A7, MSH 2
计算分子量 105 kDa
观测分子量105 kDa
GenBank蛋白编号BC021566
基因名称 MSH2
Gene ID (NCBI) 4436
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43246
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

实验方案

Product Specific Protocols
WB protocol for MSH2 antibody 60161-2-IgDownload protocol
IHC protocol for MSH2 antibody 60161-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols