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验证数据展示

  • WB analysis of mouse spleen using 25028-1-AP

    WB analysis of mouse spleen using 25028-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25028-1-AP (MSC Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 25028-1-AP

    IHC staining of human lymphoma using 25028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MSC Polyclonal antibody

MSC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25028-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

bHLHa22, Activated B-cell factor 1, Activated B cell factor 1, ABF-1, ABF1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25028-1-AP targets MSC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13489

Product name: Recombinant human MSC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-206 aa of BC006313

Sequence: MSTGSVSDPEEMELRGLQREYPVPASKRPPLRGVERSYASPSDNSSAEEEDPDGEEERCALGTAGSAEGCKRKRPRVAGGGGAGGSAGGGGKKPLPAKGSAAECKQSQRNAANARERARMRVLSKAFSRLKTSLPWVPPDTKLSKLDTLRLASSYIAHLRQLLQEDRYENGYVHPVNLTWPFVVSGRPDSDTKEVSAANRLCGTTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 musculin (activated B-cell factor-1)
别名 bHLHa22, Activated B-cell factor 1, Activated B cell factor 1, ABF-1, ABF1
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC006313
基因名称 MSC
Gene ID (NCBI) 9242
RRIDAB_2879857
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60682
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSC, also named as Musculin, is a 206 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. MSC localizes in the nucleus and as a transcription repressor is capable of inhibiting the transactivation capability of TCF3/E47. MSC may play a role in regulating antigen-dependent B-cell differentiation.

实验方案

Product Specific Protocols
IHC protocol for MSC antibody 25028-1-APDownload protocol
WB protocol for MSC antibody 25028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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