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验证数据展示

  • WB analysis using 16273-1-AP

    WB analysis using 16273-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16273-1-AP (MRPS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 16273-1-AP

    IHC staining of human ovary cancer using 16273-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16273-1-AP (MRPS6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 16273-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 16273-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16273-1-AP (MRPS6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16273-1-AP

    IF Staining of HepG2 using 16273-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPS6 antibody (16273-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

MRPS6 Polyclonal antibody

MRPS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16273-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Small ribosomal subunit protein bS6m, S6mt, RPMS6, MRP-S6, MRP S6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16273-1-AP targets MRPS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MRPS6 fusion protein Ag9345 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein S6
别名 Small ribosomal subunit protein bS6m, S6mt, RPMS6, MRP-S6, MRP S6
计算分子量 125 aa, 14 kDa
观测分子量14 kDa
GenBank蛋白编号BC010076
基因名称 MRPS6
Gene ID (NCBI) 64968
RRIDAB_3669236
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP82932
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS6 antibody 16273-1-APDownload protocol
IHC protocol for MRPS6 antibody 16273-1-APDownload protocol
IF protocol for MRPS6 antibody 16273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols