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验证数据展示

  • WB analysis using 16457-1-AP (same clone as 16457-1-PBS)

    WB analysis using 16457-1-AP (same clone as 16457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 16457-1-AP (same clone as 16457-1-PBS)

    IHC staining of human colon cancer using 16457-1-AP (same clone as 16457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MRPS35 antibody included
  • IF Staining of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

    IF Staining of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MRPS35 antibody (16457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

    IP experiment of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

    IP result of anti-MRPS35 (IP:16457-1-AP, 4ug; Detection:16457-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

MRPS35 Polyclonal antibody, PBS Only

MRPS35 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16457-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® MRPS35 Ready-To-Use IHC Kit. MRPS35 primary antibody included.

产品信息

16457-1-PBS targets MRPS35 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9430

Product name: Recombinant human MRPS35 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-323 aa of BC015862

Sequence: MAAAALPAWLSLQSRARTLRAFSTAVYSATPVPTPSLPERTPGNERPPRRKALPPRTEKMAVDQDWPSVYPVAAPFKPSAVPLPVRMGYPVKKGVPMAKEGNLELLKIPNFLHLTPVAIKKHCEALKDFCTEWPAALDSDEKCEKHFPIEIDSTDYVSSGPSVRNPRARVVVLRVKLSSLNLDDHAKKKLIKLVGERYCKTTDVLTIKTDRCPLRRQNYDYAVYLLTVLYHESWNTEEWEKSKTEADMEEYIWENSSSERNILETLLQMKAAEKNMEINKEELLGTKEIEEYKKSVVSLKNEEENENSISQYKESVKRLLNVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein S35
别名
计算分子量 323 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC015862
基因名称 MRPS35
Gene ID (NCBI) 60488
RRIDAB_2146521
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP82673
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRPS35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRPS35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. This antibody is a rabbit polyclonal antibody raised against the full length MRPS35 protein. It specifically reacts with the 37kd MRPS35protein.

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