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验证数据展示

  • IP experiment of HepG2 using 15989-1-AP

    IP experiment of HepG2 using 15989-1-AP

    IP result of anti-MRPS26 (IP:15989-1-AP, 4ug; Detection:15989-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

  • WB analysis of HepG2 using 15989-1-AP

    WB analysis of HepG2 using 15989-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15989-1-AP

    IHC staining of human liver cancer using 15989-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human skin cancer using 15989-1-AP

    IHC staining of human skin cancer using 15989-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

MRPS26 Polyclonal antibody

MRPS26 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15989-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

C20orf193, GI008, MRP S13, MRP S26, MRPS13, MRPS26, NY BR 87, RPMS13, S13mt, S26mt

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman skin cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

15989-1-AP targets MRPS26 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 MRPS26 fusion protein Ag8797 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein S26
别名 C20orf193, GI008, MRP S13, MRP S26, MRPS13, MRPS26, NY BR 87, RPMS13, S13mt, S26mt
计算分子量 205 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC013018
基因名称 MRPS26
Gene ID (NCBI) 64949
RRIDAB_10858479
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS26 antibody 15989-1-APDownload protocol
IHC protocol for MRPS26 antibody 15989-1-APDownload protocol
IP protocol for MRPS26 antibody 15989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation

Authors - Taru Hilander
View Article
mouseWB

Life Sci Alliance

Mitochondrial stress response triggered by defects in protein synthesis quality control.

Authors - Uwe Richter
View Article
humanWB

Clin Exp Immunol

Cultured lymphocytes' mitochondrial genome integrity is not altered by cladribine

Authors - Elina Järvinen
View Article