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验证数据展示

  • WB analysis using 67903-1-Ig (same clone as 67903-1-PBS)

    WB analysis using 67903-1-Ig (same clone as 67903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67903-1-Ig (MRPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67903-1-PBS in a different storage buffer formulation.

  • WB analysis using 67903-1-Ig (same clone as 67903-1-PBS)

    WB analysis using 67903-1-Ig (same clone as 67903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67903-1-Ig (MRPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67903-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67903-1-Ig (same clone as 67903-1-PBS)

    WB analysis of HepG2 using 67903-1-Ig (same clone as 67903-1-PBS)

    WB result of MRPS25 antibody (67903-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS25 transfected HepG2 cells. This data was developed using the same antibody clone with 67903-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MRPS25 Monoclonal antibody, PBS Only

MRPS25 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67903-1-PBS

产品说明书

CloneNo. 3B10B6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, Indirect ELISA

别名

DKFZp313H0817, FLJ00023, MRP S25, MRPS25, RPMS25, S25mt

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67903-1-PBS targets MRPS25 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 MRPS25 fusion protein Ag7846 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein S25
别名 DKFZp313H0817, FLJ00023, MRP S25, MRPS25, RPMS25, S25mt
计算分子量 20 kDa
观测分子量20 kDa
GenBank蛋白编号BC003590
基因名称 MRPS25
Gene ID (NCBI) 64432
RRIDAB_2918659
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP82663
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial ribosomal protein S25 (MRPS25) belongs to the ribosomal protein S25/L51 family. It is a component of the mitochondrial ribosome small subunit (28S). The variant segregated with the disease and substitutes a highly conserved proline residue with leucine (p.P72L) that, based on the high-resolution structure of the 28S ribosome, is predicted to compromise inter-protein contacts and destabilize the small subunit.