MRPS16 Polyclonal antibody

MRPS16 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 16735-1-AP

Print datasheet

Synonyms

CGI 132, COXPD2, MRP S16, MRPS16, RPMS16, S16mt



经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHEK-293 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 18 publications below

产品信息

16735-1-AP targets MRPS16 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MRPS16 fusion protein Ag9749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S16
Synonyms CGI 132, COXPD2, MRP S16, MRPS16, RPMS16, S16mt
Calculated Molecular Weight 137 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC021106
Gene Symbol MRPS16
Gene ID (NCBI) 51021
RRIDAB_2180166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3D3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS16 antibody 16735-1-APDownload protocol
IF protocol for MRPS16 antibody 16735-1-APDownload protocol
IP protocol for MRPS16 antibody 16735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Adv

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Authors - Kara L Perks
mouseWB

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler
humanWB

Nucleic Acids Res

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Authors - Elena Lavdovskaia
mouseWB

Nat Commun

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Authors - Tara R Richman
mouseWB

EMBO J

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Authors - Nicola Ferreira
mouseWB

Aging Cell

Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.

Authors - Tara R Richman