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MRPS12 Polyclonal antibody

MRPS12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15225-1-AP

Print datasheet

Synonyms

MPR S12, MRP S12, MRPS12, MT RPS12, RPMS12, RPS12, RPSM12, S12mt

验证数据展示

  • WB analysis using 15225-1-AP

    WB analysis using 15225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15225-1-AP (MRPS12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15225-1-AP

    IHC staining of human liver using 15225-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15225-1-AP (MRPS12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

15225-1-AP targets MRPS12 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen MRPS12 fusion protein Ag7442 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S12
Synonyms MPR S12, MRP S12, MRPS12, MT RPS12, RPMS12, RPS12, RPSM12, S12mt
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 13-15 kDa
GenBank Accession NumberBC001617
Gene Symbol MRPS12
Gene ID (NCBI) 6183
RRIDAB_10597843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15235
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS12 antibody 15225-1-APDownload protocol
IHC protocol for MRPS12 antibody 15225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO J

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Authors - Nicola Ferreira
View Article
mouseWB

Aging Cell

Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.

Authors - Tara R Richman
View Article
humanWB

BMC Genomics

Transcriptomic profiles of aging in purified human immune cells.

Authors - Lindsay M Reynolds
View Article
humanWB

Science

Autoregulatory control of mitochondrial glutathione homeostasis

Authors - Yuyang Liu
View Article