MRPS12 Polyclonal antibody

MRPS12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15225-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

MPR S12, MRP S12, MRPS12, MT RPS12, RPMS12, RPS12, RPSM12, S12mt

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

15225-1-AP targets MRPS12 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 MRPS12 fusion protein Ag7442 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein S12
别名 MPR S12, MRP S12, MRPS12, MT RPS12, RPMS12, RPS12, RPSM12, S12mt
计算分子量 15 kDa
观测分子量 13-15 kDa
GenBank蛋白编号BC001617
基因名称 MRPS12
Gene ID (NCBI) 6183
RRIDAB_10597843
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15235
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS12 antibody 15225-1-APDownload protocol
IHC protocol for MRPS12 antibody 15225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO J

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Authors - Nicola Ferreira
mouseWB

Aging Cell

Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.

Authors - Tara R Richman
humanWB

BMC Genomics

Transcriptomic profiles of aging in purified human immune cells.

Authors - Lindsay M Reynolds
humanWB

Science

Autoregulatory control of mitochondrial glutathione homeostasis

Authors - Yuyang Liu
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