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验证数据展示

  • IHC staining of mouse colon using 16142-1-AP

    IHC staining of mouse colon using 16142-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16142-1-AP (MRPL53 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of A549 using 16142-1-AP

    WB analysis of A549 using 16142-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16142-1-AP (MRPL53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 16142-1-AP

    IHC staining of human skin cancer using 16142-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16142-1-AP (MRPL53 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin cancer using 16142-1-AP

    IHC staining of human skin cancer using 16142-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16142-1-AP (MRPL53 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 16142-1-AP

    IF Staining of A549 using 16142-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 16142-1-AP (MRPL53 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MRPL53 Polyclonal antibody

MRPL53 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16142-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

L53mt, Large ribosomal subunit protein mL53, MRP L53, MRP-L53

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman skin cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16142-1-AP targets MRPL53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag9210

Product name: Recombinant human MRPL53 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-112 aa of BC012163

Sequence: MAAALARLGLRPVKQVRVQFCPFEKNVESTRTFLQTVSSEKVRSTNLNCSVIADVRHDGSEPCVDVLFGDGHRLIMRGAHLTALEMLTAFASHIRARDAAGSGDKPGADTGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L53
别名 L53mt, Large ribosomal subunit protein mL53, MRP L53, MRP-L53
计算分子量 112 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC012163
基因名称 MRPL53
Gene ID (NCBI) 116540
RRIDAB_2878223
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EL3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for MRPL53 antibody 16142-1-APDownload protocol
IHC protocol for MRPL53 antibody 16142-1-APDownload protocol
WB protocol for MRPL53 antibody 16142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

A roadmap for ribosome assembly in human mitochondria

Authors - Elena Lavdovskaia
View Article
humanWB

Cell Rep

A microscopy-based screen identifies cellular kinases modulating mitochondrial translation

Authors - Roya Yousefi
View Article
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