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验证数据展示

  • IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • WB analysis using 66531-1-Ig (same clone as 66531-1-PBS)

    WB analysis using 66531-1-Ig (same clone as 66531-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66531-1-Ig (same clone as 66531-1-PBS)

    WB analysis of HeLa using 66531-1-Ig (same clone as 66531-1-PBS)

    WB result of MRPL45 antibody (66531-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MRPL45 Monoclonal antibody, PBS Only

MRPL45 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66531-1-PBS

产品说明书

CloneNo. 1B4B4

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

L45mt, MRPL45, MRP-L45

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66531-1-PBS targets MRPL45 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag8207

Product name: Recombinant human MRPL45 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 86-329 aa of BC006235

Sequence: AGLVIPPEKSDRSIHLACTAGIFDAYVPPEGDARISSLSKEGLIERTERMKKTMASQVSIRRIKDYDANFKIKDFPEKAKDIFIEAHLCLNNSDHDRLHTLVTEHCFPDMTWDIKYKTVRWSFVESLEPSHVVQVRCSSMMNQGNVYGQITVRMHTRQTLAIYDRFGRLMYGQEDVPKDVLEYVVFEKQLTNPYGSWRMHTKIVPPWAPPKQPILKTVMIPGPQLKPEEEYEEAQGEAQKPQLA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L45
别名 L45mt, MRPL45, MRP-L45
计算分子量 35 kDa
观测分子量 33 kDa
GenBank蛋白编号BC006235
基因名称 MRPL45
Gene ID (NCBI) 84311
RRIDAB_2881894
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRJ2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.