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验证数据展示

  • WB analysis using 68467-1-Ig

    WB analysis using 68467-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68467-1-Ig (MRPL28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of HepG2 using 68467-1-Ig

    IF Staining of HepG2 using 68467-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL28 antibody (68467-1-Ig, Clone: 2E6B2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

MRPL28 Monoclonal antibody

MRPL28 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68467-1-Ig

产品说明书

CloneNo. 2E6B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

L28mt, Large ribosomal subunit protein bL28m, MAAT1, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH-3T3 cells, 4T1 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68467-1-Ig targets MRPL28 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MRPL28 fusion protein Ag33198 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L28
别名 L28mt, Large ribosomal subunit protein bL28m, MAAT1, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC000990
基因名称 MRPL28
Gene ID (NCBI) 10573
RRIDAB_3085178
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13084
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL28 antibody 68467-1-IgDownload protocol
IF protocol for MRPL28 antibody 68467-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols