验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, HEK-293 cells, HeLa cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
11706-1-AP targets MRPL23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2220 Product name: Recombinant human MRPL23 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-153 aa of BC027710 Sequence: MARNVVYPLYRLGGPQLRVFRTNFFIQLVRPGVAQPEDTVQFRIPMEMTRVDLRNYLEGIYNVPVAAVRTRVQHGSNKRRDHRNVRIKKPDYKVAYVQLAHGQTFTFPDLFPEKDESPEGSAADDLYSMLEEERQQRQSSDPRRGGVPSWFGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial ribosomal protein L23 |
| 别名 | MRP-L23, MRP L23, Large ribosomal subunit protein uL23m, L23mt, L23MRP |
| 计算分子量 | 153 aa, 18 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC027710 |
| 基因名称 | MRPL23 |
| Gene ID (NCBI) | 6150 |
| RRID | AB_10694422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16540 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPL23 antibody 11706-1-AP | Download protocol |
| IHC protocol for MRPL23 antibody 11706-1-AP | Download protocol |
| WB protocol for MRPL23 antibody 11706-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice. | ||
Nucleic Acids Res METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis. | ||
Aging Cell Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan. | ||
J Cell Biol Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. | ||
PLoS Genet Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction. | ||
Genomics Proteomics Bioinformatics Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses. |
