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验证数据展示

  • WB analysis using 16969-1-AP (same clone as 16969-1-PBS)

    WB analysis using 16969-1-AP (same clone as 16969-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16969-1-AP (MRPL20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16969-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16969-1-AP (same clone as 16969-1-PBS)

    IF Staining of HepG2 using 16969-1-AP (same clone as 16969-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL20 antibody (16969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16969-1-PBS in a different storage buffer formulation.

MRPL20 Polyclonal antibody, PBS Only

MRPL20 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16969-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

L20mt, Large ribosomal subunit protein bL20m, MRP L20, MRP-L20

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16969-1-PBS targets MRPL20 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9160

Product name: Recombinant human MRPL20 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-149 aa of BC009515

Sequence: MVFLTAQLWLRNRVTDRYFRIQEVLKHARHFRGRKNRCYRLAVRTVIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPALIGNLVKCQVELNRKVLADLAIYEPKTFKSLAALASRRRHEGFAAALGDGKEPEGIFSRVVQYH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L20
别名 L20mt, Large ribosomal subunit protein bL20m, MRP L20, MRP-L20
计算分子量 149 aa, 17 kDa
观测分子量17 kDa
GenBank蛋白编号BC009515
基因名称 MRPL20
Gene ID (NCBI) 55052
RRIDAB_3741901
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYC9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MRPL20 (mitochondrial ribosomal protein L20) is a 149-amino-acid nuclear-encoded protein that belongs to the bacterial ribosomal protein bL20 family. As a structural constituent of the mitochondrial ribosome , MRPL20 localizes to the 39S large subunit, where it integrates into the mitochondrial inner membrane and facilitates the synthesis of mitochondrial genome-encoded proteins-including essential transmembrane subunits of the mitochondrial respiratory chain-by participating in all three phases of mitochondrial translation: initiation, elongation, and termination.

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