验证数据展示

WB analysis using 15543-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15543-1-AP (MRPL11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 15543-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15543-1-AP (MRPL11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 15543-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15543-1-AP (MRPL11 antibody) at dilution of 1:50 (under 40x lens).
IP experiment of mouse liver using 15543-1-AP
IP result of anti-MRPL11 (IP:15543-1-AP, 4ug; Detection:15543-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IF Staining of HepG2 using 15543-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15543-1-AP (MRPL11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MRPL11 Polyclonal antibody

MRPL11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15543-1-AP

产品说明书

引用文献(15)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

MRP-L11, MRP L11, Large ribosomal subunit protein uL11m, L11mt, CGI-113

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (15)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	BxPC-3 cells, K-562 cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 15 publications below
CoIP	See 2 publications below

产品信息

15543-1-AP targets MRPL11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse
免疫原	MRPL11 fusion protein Ag7899 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	mitochondrial ribosomal protein L11
别名	MRP-L11, MRP L11, Large ribosomal subunit protein uL11m, L11mt, CGI-113
计算分子量	21 kDa
观测分子量	21 kDa
GenBank蛋白编号	BC005002
基因名称	MRPL11
Gene ID (NCBI)	65003
RRID	AB_2297856
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9Y3B7
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

MRPL11 is one of the protein components of mitochondrial ribosomes encoded by the nuclear genome, which is essential for oxidative phosphorylation.

实验方案

Product Specific Protocols
WB protocol for MRPL11 antibody 15543-1-AP	Download protocol
IHC protocol for MRPL11 antibody 15543-1-AP	Download protocol
IF protocol for MRPL11 antibody 15543-1-AP	Download protocol
IP protocol for MRPL11 antibody 15543-1-AP	Download protocol
FC protocol for MRPL11 antibody 15543-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Sci Adv Adult-onset obesity is triggered by impaired mitochondrial gene expression. Authors - Kara L Perks View Article
human	WB	Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. Authors - Uwe Richter View Article
mouse	WB	J Cell Biol Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. Authors - Uwe Richter View Article
human	WB	Am J Hum Genet Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome. Authors - Nicole J Lake View Article
human	WB	Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation Authors - Liang Wang View Article
human	WB	Hum Mol Genet Translation of MT-ATP6 pathogenic variants reveals distinct regulatory consequences from the co-translational quality control of mitochondrial protein synthesis. Authors - Kah Ying Ng View Article

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验证数据展示

WB analysis using 15543-1-AP

IHC staining of human liver using 15543-1-AP

IHC staining of human liver using 15543-1-AP

IP experiment of mouse liver using 15543-1-AP

IF Staining of HepG2 using 15543-1-AP

MRPL11 Polyclonal antibody

MRPL11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Translation of MT-ATP6 pathogenic variants reveals distinct regulatory consequences from the co-translational quality control of mitochondrial protein synthesis.