MRPL11 Polyclonal antibody
MRPL11 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, K-562 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 12 publications below |
| CoIP | See 1 publications below |
产品信息
15543-1-AP targets MRPL11 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB,ELISA |
| Cited Applications | WB, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MRPL11 fusion protein Ag7899 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitochondrial ribosomal protein L11 |
| Synonyms | CGI 113, L11MT, MRP L11, MRPL11 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC005002 |
| Gene Symbol | MRPL11 |
| Gene ID (NCBI) | 65003 |
| RRID | AB_2297856 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3B7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MRPL11 is one of the protein components of mitochondrial ribosomes encoded by the nuclear genome, which is essential for oxidative phosphorylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL11 antibody 15543-1-AP | Download protocol |
| IHC protocol for MRPL11 antibody 15543-1-AP | Download protocol |
| IF protocol for MRPL11 antibody 15543-1-AP | Download protocol |
| IP protocol for MRPL11 antibody 15543-1-AP | Download protocol |
| FC protocol for MRPL11 antibody 15543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. | ||
J Cell Biol Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. | ||
Am J Hum Genet Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome. | ||
Hum Mol Genet Translation of MT-ATP6 pathogenic variants reveals distinct regulatory consequences from the co-translational quality control of mitochondrial protein synthesis. | ||
Hum Mol Genet A variant in MRPS14 (uS14m) causes perinatal hypertrophic cardiomyopathy with neonatal lactic acidosis, growth retardation, dysmorphic features and neurological involvement. |