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验证数据展示

  • WB analysis of HT-1080 using 19916-1-AP

    WB analysis of HT-1080 using 19916-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 19916-1-AP

    IHC staining of rat kidney using 19916-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 19916-1-AP

    IHC staining of rat kidney using 19916-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRI1 Polyclonal antibody

MRI1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19916-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

M1Pi, MRDI, MRI1, MTNA, MTR 1 P isomerase, Ypr118w

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, HL-60 cells
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19916-1-AP targets MRI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MRI1 fusion protein Ag13809 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methylthioribose-1-phosphate isomerase homolog (S. cerevisiae)
别名 M1Pi, MRDI, MRI1, MTNA, MTR 1 P isomerase, Ypr118w
计算分子量 369 aa, 39 kDa
观测分子量 45 kDa
GenBank蛋白编号BC001703
基因名称 MRI1
Gene ID (NCBI) 84245
RRIDAB_10638924
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV20
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRI1 antibody 19916-1-APDownload protocol
IHC protocol for MRI1 antibody 19916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols