MRFAP1 Polyclonal antibody

MRFAP1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11639-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, IHC, ELISA and More (1)

MRFAP1, PAM14, PGR1, Protein PGR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11639-1-AP targets MRFAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IP, IF
经测试反应性 human
文献引用反应性human, monkey
免疫原 MRFAP1 fusion protein Ag2227 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Mof4 family associated protein 1
别名 MRFAP1, PAM14, PGR1, Protein PGR1
计算分子量 127 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC022797
基因名称 MRFAP1
Gene ID (NCBI) 93621
RRIDAB_2145271
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y605
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRFAP1, also named as PAM14 and PGR1, is a 14 kDa protein which associated with MRG. It is found in a complex composed of MORF4L1, MRFAP1 and RB1. MRFAP1 interacts via its N-terminus with MORF4L1. And it interacts with CSTB and MORF4L2.

实验方案

Product Specific Protocols
WB protocol for MRFAP1 antibody 11639-1-APDownload protocol
IHC protocol for MRFAP1 antibody 11639-1-APDownload protocol
IP protocol for MRFAP1 antibody 11639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Characterisation of Native Protein Complexes and Protein Isoform Variation using Size-Fractionation Based Quantitative Proteomics.

Authors - Kirkwood Kathryn J KJ
humanWB

Mol Cell Proteomics

Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924.

Authors - Hua Liao
human,monkeyWB,IP

J Cell Biochem

Interaction of Sedlin with PAM14.

Authors - Liu Xiaoying X
humanIF, WB

Oncotarget

FBXW8-dependent degradation of MRFAP1 in anaphase controls mitotic cell death.

Authors - Duan-Zhuo Li
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