验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11639-1-AP targets MRFAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IP, IF |
经测试反应性 | human |
文献引用反应性 | human, monkey |
免疫原 | MRFAP1 fusion protein Ag2227 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Mof4 family associated protein 1 |
别名 | MRFAP1, PAM14, PGR1, Protein PGR1 |
计算分子量 | 127 aa, 15 kDa |
观测分子量 | 15 kDa |
GenBank蛋白编号 | BC022797 |
基因名称 | MRFAP1 |
Gene ID (NCBI) | 93621 |
RRID | AB_2145271 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y605 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MRFAP1, also named as PAM14 and PGR1, is a 14 kDa protein which associated with MRG. It is found in a complex composed of MORF4L1, MRFAP1 and RB1. MRFAP1 interacts via its N-terminus with MORF4L1. And it interacts with CSTB and MORF4L2.
实验方案
Product Specific Protocols | |
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WB protocol for MRFAP1 antibody 11639-1-AP | Download protocol |
IHC protocol for MRFAP1 antibody 11639-1-AP | Download protocol |
IP protocol for MRFAP1 antibody 11639-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Proteomics Characterisation of Native Protein Complexes and Protein Isoform Variation using Size-Fractionation Based Quantitative Proteomics. | ||
Mol Cell Proteomics Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. | ||
Oncotarget FBXW8-dependent degradation of MRFAP1 in anaphase controls mitotic cell death. |