验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11639-1-AP targets MRFAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, monkey |
| 免疫原 |
CatNo: Ag2227 Product name: Recombinant human MRFAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-127 aa of BC022797 Sequence: MRPLDIVELAEPEEVEVLEPEEDFEQFLLPVINEMREDIASLTREHGRAYLRNRSKLWEMDNMLIQIKTQVEASEESALNHLQNPGDAAEGRAAKRCEKAEEKAKEIAKMAEMLVELVRRIEKSESS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Mof4 family associated protein 1 |
| 别名 | MRFAP1, PAM14, PGR1, Protein PGR1 |
| 计算分子量 | 127 aa, 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC022797 |
| 基因名称 | MRFAP1 |
| Gene ID (NCBI) | 93621 |
| RRID | AB_2145271 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y605 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MRFAP1, also named as PAM14 and PGR1, is a 14 kDa protein which associated with MRG. It is found in a complex composed of MORF4L1, MRFAP1 and RB1. MRFAP1 interacts via its N-terminus with MORF4L1. And it interacts with CSTB and MORF4L2.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MRFAP1 antibody 11639-1-AP | Download protocol |
| IP protocol for MRFAP1 antibody 11639-1-AP | Download protocol |
| WB protocol for MRFAP1 antibody 11639-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Characterisation of Native Protein Complexes and Protein Isoform Variation using Size-Fractionation Based Quantitative Proteomics. | ||
Mol Cell Proteomics Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. | ||
Oncotarget FBXW8-dependent degradation of MRFAP1 in anaphase controls mitotic cell death. |
