验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14207-1-AP targets MREG in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag5416 Product name: Recombinant human MREG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-214 aa of BC032747 Sequence: MGLRDWLRTVCCCCGCECLEERALPEKEPLVSDNNPYSSFGATLVRDDEKNLWSMPHDVSHTEADDDRTLYNLIVIRNQQAKDSEEWQKLNYDIHTLRQVRREVRNRWKCILEDLGFQKEADSLLSVTKLSTISDSKNTRKAREMLLKLAEETNIFPTSWELSERYLFVVDRLIALDAAEEFFKLARRTYPKKPGVPCLADGQKELHYLPFPSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | melanoregulin |
| 别名 | DSU, melanoregulin, MREG, WDT2 |
| 计算分子量 | 25kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC032747 |
| 基因名称 | MREG |
| Gene ID (NCBI) | 55686 |
| RRID | AB_3085432 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N565 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MREG, also known as DSU, belongs to the melanoregulin family. MREG is a small, highly charged protein thought to play a role in organelle biogenesis due to its effect on pigmentation in dilute, ashen, and leaden mutant mice (PMID: 19240024). Additionally, MREG probably functions as a cargo-recognition protein that couples cytoplasmic vesicles to the transport machinery. MREG has 2 isoforms with the molecular weights of 25 and 26 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MREG antibody 14207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
