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CD206 Recombinant antibody

CD206 Recombinant Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig, Mouse

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G15

Cat No : 81525-1-RR

Print datasheet

Synonyms

CD206, CLEC13D, Macrophage mannose receptor 1, mannose receptor, C type 1, MMR, MRC1

验证数据展示

  • WB analysis using 81525-1-RR

    WB analysis using 81525-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81525-1-RR (CD206 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 81525-1-RR

    IHC staining of human lung cancer using 81525-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD206 antibody included
  • IHC staining of human lung cancer using 81525-1-RR

    IHC staining of human lung cancer using 81525-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD206 antibody included
  • IF Staining of mouse lung using 81525-1-RR

    IF Staining of mouse lung using 81525-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse lung using 81525-1-RR

    IF Staining of mouse lung using 81525-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue, pig liver tissue
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse lung tissue
Planning an IHC experiment? We recommend our IHCeasy CD206 Ready-To-Use IHC Kit. CD206 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81525-1-RR targets CD206 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human, Pig, Mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mannose receptor, C type 1
Synonyms CD206, CLEC13D, Macrophage mannose receptor 1, mannose receptor, C type 1, MMR, MRC1
Calculated Molecular Weight 166 kDa
Observed Molecular Weight170 kDa
GenBank Accession NumberNM_002438
Gene Symbol MRC1
Gene ID (NCBI) 4360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22897
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

实验方案

Product Specific Protocols
WB protocol for CD206 antibody 81525-1-RRDownload protocol
IHC protocol for CD206 antibody 81525-1-RRDownload protocol
IF protocol for CD206 antibody 81525-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols