验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HaCaT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11787-1-AP targets MPZL2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MPZL2 fusion protein Ag2419 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myelin protein zero-like 2 |
| 别名 | Epithelial V like antigen 1, EVA, EVA1, MPZL2, myelin protein zero like 2 |
| 计算分子量 | 215 aa, 24 kDa |
| 观测分子量 | 24-30 kDa |
| GenBank蛋白编号 | BC017774 |
| 基因名称 | MPZL2 |
| Gene ID (NCBI) | 10205 |
| RRID | AB_2282054 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60487 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MPZL2 (myelin protein zero-like 2), also named as EVA (epithelial V-like antigen) or EVA1, is a type 1 transmembrane protein of the myelin P0 protein family, containing an Ig-like V-type (immunoglobulin-like) domain and two potential glycosylation sites. MPZL2 is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression. Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MPZL2 antibody 11787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Hearing restoration by gene replacement therapy for a multisite-expressed gene in a mouse model of human DFNB111 deafness
| ||
Am J Hum Genet MPZL2, Encoding the Epithelial Junctional Protein Myelin Protein Zero-like 2, Is Essential for Hearing in Man and Mouse. | ||
PLoS Pathog Haploid Genetic Screens Identify an Essential Role for PLP2 in the Downregulation of Novel Plasma Membrane Targets by Viral E3 Ubiquitin Ligases. | ||
Cancer Sci Epithelial V-like antigen 1 promotes hepatocellular carcinoma growth and metastasis via the ERBB-PI3K-AKT pathway.
| ||
Hum Genet MPZL2 is a novel gene associated with autosomal recessive nonsyndromic moderate hearing loss. |