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MPPED2 Polyclonal antibody

MPPED2 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13270-1-AP

Print datasheet

Synonyms

239FB, C11orf8, FAM1B, Fetal brain protein 239, Metallophosphoesterase MPPED2, MPPED2

验证数据展示

  • WB analysis of human brain using 13270-1-AP

    WB analysis of human brain using 13270-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13270-1-AP (MPPED2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13270-1-AP

    IF Staining of HepG2 using 13270-1-AP

    Immunofluorescent analysis of HepG2 cells, using MPPED2 antibody 13270-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human brain using 13270-1-AP

    IHC staining of human brain using 13270-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13270-1-AP (MPPED2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 13270-1-AP

    IHC staining of human brain using 13270-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13270-1-AP (MPPED2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13270-1-AP targets MPPED2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human
Immunogen MPPED2 fusion protein Ag3963 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name metallophosphoesterase domain containing 2
Synonyms 239FB, C11orf8, FAM1B, Fetal brain protein 239, Metallophosphoesterase MPPED2, MPPED2
Calculated Molecular Weight 294 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC031582
Gene Symbol MPPED2
Gene ID (NCBI) 744
RRIDAB_2144517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15777
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MPPED2 antibody 13270-1-APDownload protocol
IHC protocol for MPPED2 antibody 13270-1-APDownload protocol
IF protocol for MPPED2 antibody 13270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols