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  • Featured Product
  • KD/KO Validated

MPP7 Polyclonal antibody

MPP7 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12983-1-AP

Print datasheet

Synonyms

MAGUK p55 subfamily member 7, MPP7

验证数据展示

  • WB analysis of HeLa using 12983-1-AP

    WB analysis of HeLa using 12983-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12983-1-AP (MPP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 12983-1-AP

    IHC staining of human ovary tumor using 12983-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12983-1-AP (MPP7 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HeLa using 12983-1-AP

    IP experiment of HeLa using 12983-1-AP

    IP result of anti-MPP7 (IP:12983-1-AP, 4ug; Detection:12983-1-AP 1:500) with HeLa cells lysate 2800ug.

经过测试的应用

Positive WB detected inHeLa cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12983-1-AP targets MPP7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MPP7 fusion protein Ag3612 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7)
Synonyms MAGUK p55 subfamily member 7, MPP7
Calculated Molecular Weight 576 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC038105
Gene Symbol MPP7
Gene ID (NCBI) 143098
RRIDAB_2291710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T2T1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MPP7 antibody 12983-1-APDownload protocol
IHC protocol for MPP7 antibody 12983-1-APDownload protocol
IP protocol for MPP7 antibody 12983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Hum Mol Genet

Post-genome wide association studies and functional analyses identify association of MPP7 gene variants with site-specific bone mineral density.

Authors - Xiao Su-Mei SM
View Article
humanIHC,WB

Cell Biol Int

MPP7 promotes the migration and invasion of breast cancer cells via EGFR/AKT signaling.

Authors - Wanqin Liao
  • KD Validated
View Article
humanWB,IHC

Life (Basel)

MPP7 as a Novel Biomarker of Esophageal Cancer: MPP7 Knockdown Inhibits Esophageal Cancer Cell Migration and Invasion

Authors - Zhaodong Li
  • KD Validated
View Article