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验证数据展示

  • WB analysis using 17710-1-AP (same clone as 17710-1-PBS)

    WB analysis using 17710-1-AP (same clone as 17710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

    IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

    IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

    IP experiment of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

    IP result of anti-MPP5 (IP:17710-1-AP, 3ug; Detection:17710-1-AP 1:1000) with mouse brain tissue lysate 8000ug. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 17710-1-AP (same clone as 17710-1-PBS)

    IF Staining of HEK-293 using 17710-1-AP (same clone as 17710-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MPP5 antibody (17710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MPP5 Polyclonal antibody, PBS Only

MPP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17710-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17710-1-PBS targets MPP5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12010

Product name: Recombinant human PALS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-341 aa of BC095485

Sequence: MTTSHMNGHVTEESDSEVKNVDLASPEEHQKHREMAVDCPGDLGTRMMPIRRSAQLERIRQQQEDMRRRREEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPMFDTEEGIVLESPHYAVKILEIEDLFSSLKHIQHTLVDSQSQEDISLLLQLVQNKDFQNAFKIHNAITVHMNKASPPFPLISNAQDLAQEVQTVLKPVHHKEGQELTALLNTPHIQALLLAHDKVAEQEMQLEPITDERVYESIGQYGGETVKIVRIEKARDIPLGATVRNEMDSVIISRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
别名
计算分子量 675 aa, 77 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC095485
基因名称 MPP5
Gene ID (NCBI) 64398
RRIDAB_2282012
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N3R9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.

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