• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse eye using 13589-1-AP

    WB analysis of mouse eye using 13589-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13589-1-AP

    WB analysis using 13589-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse eye using 13589-1-AP

    IP experiment of mouse eye using 13589-1-AP

    IP result of anti-MPP2 (IP:13589-1-AP, 4ug; Detection:13589-1-AP 1:2000) with mouse eye tissue lysate 2260 ug.

  • IHC staining of mouse brain using 13589-1-AP

    IHC staining of mouse brain using 13589-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13589-1-AP

    IHC staining of mouse brain using 13589-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MPP2 Polyclonal antibody

MPP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13589-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

Discs large homolog 2, DLG2, MAGUK p55 subfamily member 2, MPP2, Protein MPP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse eye tissue, mouse retina tissue
Positive IP detected inmouse eye tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13589-1-AP targets MPP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MPP2 fusion protein Ag4223 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
别名 Discs large homolog 2, DLG2, MAGUK p55 subfamily member 2, MPP2, Protein MPP2
计算分子量 576 aa, 65 kDa
观测分子量 65 kDa, 60 kDa
GenBank蛋白编号BC030287
基因名称 MPP2
Gene ID (NCBI) 4355
RRIDAB_2935434
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14168
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Membrane protein palmitoylated 2 (MPP2) is a member of the membrane-associated guanylate kinase (MAGUK) family. MPPs can form diverse protein complexes at the cell membranes and play an important role in cell structure, polarity and cell adhesion. MPP2 is a novel postsynaptic scaffold protein that binds to the synaptic cell adhesion molecule SynCAM1. MPP2 also is a component of AMPA receptor-associated protein complexes.

实验方案

Product Specific Protocols
WB protocol for MPP2 antibody 13589-1-APDownload protocol
IHC protocol for MPP2 antibody 13589-1-APDownload protocol
IP protocol for MPP2 antibody 13589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols