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验证数据展示

  • WB analysis using 84436-5-RR (same clone as 84436-5-PBS)

    WB analysis using 84436-5-RR (same clone as 84436-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84436-5-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84436-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84436-5-RR (same clone as 84436-5-PBS)

    IHC staining of mouse spleen using 84436-5-RR (same clone as 84436-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84436-5-RR (MPO antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84436-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 84436-5-RR (same clone as 84436-5-PBS)

    IF Staining of mouse spleen using 84436-5-RR (same clone as 84436-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using MPO antibody (84436-5-RR, Clone: 241839C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84436-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84436-5-RR

    Affinity and Kinetic Characterization of 84436-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84436-5-RR against Mouse Mpo were performed. The affinity constant is 23.5 nM.

Mouse MPO Recombinant antibody, PBS Only

MPO Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84436-5-PBS

产品说明书

CloneNo. 241839C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Myeloperoxidase light chain, Myeloperoxidase heavy chain, Myeloperoxidase, EC:1.11.2.2, 241839C11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84436-5-PBS targets MPO in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myeloperoxidase
别名 Myeloperoxidase light chain, Myeloperoxidase heavy chain, Myeloperoxidase, EC:1.11.2.2, 241839C11
计算分子量81KD
观测分子量 43 kDa, 59 kDa, 90 kDa
GenBank蛋白编号AAR99349
基因名称 Mpo
Gene ID (NCBI) 17523
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP11247
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.