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验证数据展示

  • WB analysis using 85818-5-RR

    WB analysis using 85818-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85818-5-RR (MOV10 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85818-5-RR

    IF Staining of HepG2 using 85818-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MOV10 antibody (85818-5-RR, Clone: 250130D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85818-5-RR

    Affinity and Kinetic Characterization of 85818-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85818-5-RR against Human MOV10 were performed. The affinity constant is below 1 pM.

MOV10 Recombinant antibody

MOV10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85818-5-RR

产品说明书

CloneNo. 250130D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Armitage homolog, EC:3.6.4.13, fSAP113, gb110, Helicase MOV-10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, L02 cells, MCF-7 cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MOV10 Ready-To-Use IHC Kit. MOV10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85818-5-RR targets MOV10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MOV10 fusion protein Ag0384 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Mov10, Moloney leukemia virus 10, homolog (mouse)
别名 Armitage homolog, EC:3.6.4.13, fSAP113, gb110, Helicase MOV-10
计算分子量 114 kDa
观测分子量 115 kDa
GenBank蛋白编号BC002548
基因名称 MOV10
Gene ID (NCBI) 4343
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HCE1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals (PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages (PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

实验方案

Product Specific Protocols
WB protocol for MOV10 antibody 85818-5-RRDownload protocol
IF protocol for MOV10 antibody 85818-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols