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验证数据展示

  • WB analysis using 25617-1-AP

    WB analysis using 25617-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25617-1-AP (MOSPD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 25617-1-AP

    WB analysis of HeLa using 25617-1-AP

    WB result of MOSPD2 antibody (25617-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MOSPD2 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

MOSPD2 Polyclonal antibody

MOSPD2 Polyclonal Antibody for WB, ELISA
Cat No. 25617-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Motile sperm domain-containing protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHuH-7 cells, HeLa cells, J774A.1 cells, RAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy MOSPD2 Ready-To-Use IHC Kit. MOSPD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25617-1-AP targets MOSPD2 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22206

Product name: Recombinant human MOSPD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 64-496 aa of BC030641

Sequence: MLDESFQWRKEISVNDLNESSIPRWLLEIGVIYLHGYDKEGNKLFWIRVKYHVKDQKTILDKKKLIAFWLERYAKRENGKPVTVMFDLSETGINSIDMDFVRFIINCFKVYYPKYLSKIVIFDMPWLMNAAFKIVKTWLGPEAVSLLKFTSKNEVQDYVSVEYLPPHMGGTDPFKYSYPPLVDDDFQTPLCENGPITSEDETSSKEDIESDGKETLETISNEEQTPLLKKINPTESTSKAEENEKVDSKVKAFKKPLSVFKGPLLHISPAEELYFGSTESGEKKTLIVLTNVTKNIVAFKVRTTAPEKYRVKPSNSSCDPGASVDIVVSPHGGLTVSAQDRFLIMAAEMEQSSGTGPAELTQFWKEVPRNKVMEHRLRCHTVESSKPNTLTLKDNAFNMSDKTSEDICLQLSRLLESNRKLEDQVQRCIWFQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 motile sperm domain containing 2
别名 Motile sperm domain-containing protein 2
计算分子量 518 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC030641
基因名称 MOSPD2
Gene ID (NCBI) 158747
RRIDAB_3742155
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NHP6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOSPD2 is a 518-amino acid protein containing an N-terminal CRAL/TRIO domain, a major sperm protein (MSP) domain, and a potential C-terminal transmembrane domain. MOSPD2 is a member of the vesicle-associated membrane protein-associated protein (VAP) family and one of the endoplasmic reticulum (ER) receptors that function as tether proteins to bridge ER and other organelles to build contacts(PMID: 29858488, 35389430).

实验方案

Product Specific Protocols
WB protocol for MOSPD2 antibody 25617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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