验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IF | See 1 publications below |
产品信息
24994-1-AP targets MORC3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | MORC3 fusion protein Ag21825 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | MORC family CW-type zinc finger 3 |
别名 | ZCWCC3, ZCW5, NXP2, Nuclear matrix protein 2, MORC family CW-type zinc finger protein 3 |
计算分子量 | 939 aa, 107 kDa |
观测分子量 | 130 kDa |
GenBank蛋白编号 | BC132731 |
基因名称 | MORC3 |
Gene ID (NCBI) | 23515 |
RRID | AB_2879836 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q14149 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MORC3, also named as MORC family CW-type zinc finger protein 3 or NXP2, is a 939 amino acid protein, which contains 1 CW-type zinc finger. MORC3 localizes in the nucleus and are expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney. MORC3 as Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. Sumoylated MORC3-NBs can also associate with PML-NBs and recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity. It may be required for influenza A transcription during viral infection. The calculated molecular weight of MORC3 is a 107 kDa, but the Sumoylated MORC3 protein is about 130 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for MORC3 antibody 24994-1-AP | Download protocol |
IF protocol for MORC3 antibody 24994-1-AP | Download protocol |
IP protocol for MORC3 antibody 24994-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Med Virol MORC3 restricts human cytomegalovirus infection by suppressing the major immediate-early promoter activity. | ||
iScience The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis |